File d= new File(dir+"/data/human_magnus/rna/cuff/time-5");
File f= new File(d+"/novel_genes.fpkm_tracking.bed");
File of= new File(d+"/refine-"+ side+"_"+ f.getName() );
GenomicRegionsBuilder gb= new BedReader(f).getGenomicRegionsBuilder();
GenomicRegionsBuilder gbref= new BedReader(new File(dir+"/anno/hg19_ensGene_exon.bed")).getGenomicRegionsBuilder();
GenomicRegionsBuilder gbrefs= new GenomicRegionsModifier(gbref).getExtendedRegions(side, side);
GenomicRegionsComparator gc= new GenomicRegionsComparator(gb, gbrefs);
new BedExporter( gc.getNonIntersection() ).writeToFile(of);