/*
* The MIT License
*
* Copyright (c) 2010 The Broad Institute
*
* Permission is hereby granted, free of charge, to any person obtaining a copy
* of this software and associated documentation files (the "Software"), to deal
* in the Software without restriction, including without limitation the rights
* to use, copy, modify, merge, publish, distribute, sublicense, and/or sell
* copies of the Software, and to permit persons to whom the Software is
* furnished to do so, subject to the following conditions:
*
* The above copyright notice and this permission notice shall be included in
* all copies or substantial portions of the Software.
*
* THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR
* IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY,
* FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE
* AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER
* LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM,
* OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN
* THE SOFTWARE.
*/
package picard.reference;
import htsjdk.samtools.reference.ReferenceSequence;
import htsjdk.samtools.reference.ReferenceSequenceFile;
import htsjdk.samtools.reference.ReferenceSequenceFileFactory;
import htsjdk.samtools.util.CloserUtil;
import htsjdk.samtools.util.IOUtil;
import htsjdk.samtools.util.Interval;
import htsjdk.samtools.util.IntervalList;
import htsjdk.samtools.util.SequenceUtil;
import picard.PicardException;
import picard.cmdline.CommandLineProgram;
import picard.cmdline.CommandLineProgramProperties;
import picard.cmdline.Option;
import picard.cmdline.programgroups.Fasta;
import picard.cmdline.StandardOptionDefinitions;
import java.io.BufferedWriter;
import java.io.File;
import java.io.IOException;
/**
* Simple command line program that allows sub-sequences represented by an interval
* list to be extracted from a reference sequence file.
*
* @author Tim Fennell
*/
@CommandLineProgramProperties(
usage = "Extracts one or more intervals described in an interval_list file " +
"from a given reference sequence and writes them out in FASTA format. Requires a fasta index " +
"file to be present.",
usageShort = "Extracts intervals from a reference sequence, writing them to a FASTA file",
programGroup = Fasta.class
)
public class ExtractSequences extends CommandLineProgram {
@Option(doc="Interval list describing intervals to be extracted from the reference sequence.")
public File INTERVAL_LIST;
@Option(shortName= StandardOptionDefinitions.REFERENCE_SHORT_NAME, doc="Reference sequence file.")
public File REFERENCE_SEQUENCE;
@Option(shortName=StandardOptionDefinitions.OUTPUT_SHORT_NAME, doc="Output fasta file.")
public File OUTPUT;
@Option(doc="Maximum line length for sequence data.")
public int LINE_LENGTH = 80;
public static void main(final String[] args) {
new ExtractSequences().instanceMainWithExit(args);
}
@Override
protected int doWork() {
IOUtil.assertFileIsReadable(INTERVAL_LIST);
IOUtil.assertFileIsReadable(REFERENCE_SEQUENCE);
IOUtil.assertFileIsWritable(OUTPUT);
final IntervalList intervals = IntervalList.fromFile(INTERVAL_LIST);
final ReferenceSequenceFile ref = ReferenceSequenceFileFactory.getReferenceSequenceFile(REFERENCE_SEQUENCE);
SequenceUtil.assertSequenceDictionariesEqual(intervals.getHeader().getSequenceDictionary(), ref.getSequenceDictionary());
final BufferedWriter out = IOUtil.openFileForBufferedWriting(OUTPUT);
for (final Interval interval : intervals) {
final ReferenceSequence seq = ref.getSubsequenceAt(interval.getSequence(), interval.getStart(), interval.getEnd());
final byte[] bases = seq.getBases();
if (interval.isNegativeStrand()) SequenceUtil.reverseComplement(bases);
try {
out.write(">");
out.write(interval.getName());
out.write("\n");
for (int i=0; i<bases.length; ++i) {
if (i > 0 && i % LINE_LENGTH == 0) out.write("\n");
out.write(bases[i]);
}
out.write("\n");
}
catch (IOException ioe) {
throw new PicardException("Error writing to file " + OUTPUT.getAbsolutePath(), ioe);
}
}
CloserUtil.close(out);
return 0;
}
}