Package net.sf.mzmine.modules.peaklistmethods.identification.mascot.data

Examples of net.sf.mzmine.modules.peaklistmethods.identification.mascot.data.Peptide


      double ionScore = Double.parseDouble(tokens[7]);

      if (ionScore >= identityThreshold)
        continue;

      Peptide peptide = new Peptide(queryNumber, sequence, ionScore,
          mass, massExpected, precursorCharge, precursorMass,
          deltaMass, missedCleavages, null, "Mascot", isTopScore);

      HashMap<Integer, ModificationPeptide> modifications = new HashMap<Integer, ModificationPeptide>();
      ModificationPeptide[] searchedMods = pepDataFile
          .getSearchedModifications();

      // TODO Verify the possibility of two modifications in the same
      // site.
      for (int pos = 0; pos < modSeries.length(); pos++) {
        if (modSeries.charAt(pos) == '1') {
          char aa = sequence.charAt(pos);
          for (int index = 0; index < searchedMods.length; index++) {
            if (searchedMods[index].getSite() == aa) {
              modifications.put(pos, searchedMods[index]);
            }
          }
        }
      }

      peptide.setModifications(modifications);

      // Ion serie
      PeptideIonSerie peptideIonSerie = parseIonSeriesSignificance(tokens[8]);
      peptide.setIonSeries(peptideIonSerie);

      // Calculate fragmentation
      PeptideFragmentation fragmentation = new PeptideFragmentation(
          peptide, pepDataFile);
      peptide.setFragmentation(fragmentation);

      // Protein info
      ProteinSection section;
      tokens = proteinInfos[pepIndex].split(":");
      String sysname = tokens[0].replace("\"", "");

      Protein protein = pepDataFile.getProtein(sysname);
      if (protein == null)
        protein = new Protein(sysname);

      int startRegion = Integer.parseInt(tokens[2]);
      int stopRegion = Integer.parseInt(tokens[3]);
      int multiplicity = Integer.parseInt(tokens[4]);
      section = new ProteinSection(startRegion, stopRegion, multiplicity);

      // Link peptide and protein
      peptide.setProtein(protein);
      protein.addPeptide(peptide, section, isTopScore);

      peptides.add(peptide);

    }
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